Frequently Asked Questions
Q: How to use HybridKla webserver?
A: Follow these steps:
- Find the prediction website in "Sequence Protein Prediction" or "Sequence Peptide Prediction" pages of HybridKla webserver.
- Enter protein sequence(s) in fasta format (starts with '>' followed by protein/peptide name)
- Click the "Submit" button
- Wait for the prediction results of Lysine lactylation
- You can download the prediction results in csv or txt format
Q: How to read the HybridKla web server results?
A:Using the human protein P23284 as an example, here's how to interpret the results
| Protein Name | The name/id of the input protein sequence |
| Site | The position of the predicted lactylation site |
| Predict | HybridKla score evaluating lactylation potential |
| Cutoff | Threshold cutoff value affecting precision, sensitivity and specificity |
Q: How to choose the cut-off values and the thresholds?
A: The three thresholds of HybridKla were decided based on calculated FPRs.The high, medium and low thresholds were established with FPRs of 2%, 6% and 10%.